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Western blot analysis of extracts of Raji cells, using CD27 antibody (A1945) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
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Western blot analysis of extracts of various cell lines, using CD27 antibody (A1945) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
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Western blot analysis of extracts of Rat lung, using CD27 antibody (A1945) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat spleen using CD27 antibody (A1945) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using CD27 antibody (A1945) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spleen using CD27 antibody (A1945) at dilution of 1:100 (40x lens).
CD27 Rabbit pAb
Catalog No：AB-A1945
Manufacturer：ABclonal
Synonyms：CD27;S152;S152. LPFS2;T14;TNFRSF7;Tp55;S152.LPFS2
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | CD27 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-191 of human CD27 (NP_001233.1). |
| Gene ID: | 939 |
| Swiss prot: | P26842 |
| Molecular weight: | 29kDa |
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