Western blot analysis of extracts of various cell lines, using Cathepsin L/V/K/H antibody (A12200) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Cathepsin L/V/K/H Rabbit pAb
Catalog No:AB-A12200
Manufacturer:ABclonal
Synonyms:CTSL;CATL;CTSL1;MEP
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Cathepsin L/V/K/H Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Multiple alternatively spliced transcript variants have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 114-333 of human Cathepsin L/V/K/H (NP_001903.1). |
Gene ID: | 1514 |
Swiss prot: | P07711 |
Molecular weight: | 37kDa |
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