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Western blot analysis of extracts of H460 cells, using CAST antibody (A0097) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat heart using CAST antibody (A0097) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human prostate using CAST antibody (A0097) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using CAST antibody (A0097) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using CAST antibody (A0097) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using CAST antibody (A0097) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CAST antibody (A0097) at dilution of 1:100 (40x lens).
CAST Rabbit pAb
Catalog No：AB-A0097
Manufacturer：ABclonal
Synonyms：CAST;BS-17;PLACK
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | CAST Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 600 to the C-terminus of human CAST (NP_001177371.1). |
| Gene ID: | 831 |
| Swiss prot: | P20810 |
| Molecular weight: | 63kDa/71- 84kDa |
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