Western blot analysis of extracts of various cell lines, using CASP8AP2 antibody (A15770) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
CASP8AP2 Rabbit pAb
Catalog No:AB-A15770
Manufacturer:ABclonal
Synonyms:CASP8AP2;CED-4;FLASH;RIP25
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | CASP8AP2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This protein is highly similar to FLASH, a mouse apoptotic protein identified by its interaction with the death-effector domain (DED) of caspase 8. Studies of FLASH protein suggested that this protein may be a component of the death-inducing signaling complex that includes Fas receptor, Fas-binding adapter FADD, and caspase 8, and plays a regulatory role in Fas-mediated apoptosis. Alternative splicing results in multiple transcript variants encoding the same protein. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1733-1982 of human CASP8AP2 (NP_036247.1). |
Gene ID: | 9994 |
Swiss prot: | Q9UKL3 |
Molecular weight: | 222kDa |
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