Western blot analysis of extracts of various cell lines, using CAPN3 Antibody (A11995) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
CAPN3 Rabbit pAb
Catalog No:AB-A11995
Manufacturer:ABclonal
Synonyms:CAPN3;CANP3;CANPL3;LGMD2;LGMD2A;nCL-1;p94;calpain-3
Applications:WB
Reactivity:Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | CAPN3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human CAPN3 (NP_775111.1). |
Gene ID: | 825 |
Swiss prot: | P20807 |
Molecular weight: | 18kDa/35kDa/84kDa/93kDa/94kDa |
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