Western blot analysis of extracts of various cell lines, using CALML5 antibody (A7808) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
CALML5 Rabbit pAb
Catalog No:AB-A7808
Manufacturer:ABclonal
Synonyms:CALML5;CLSP
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | CALML5 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a novel calcium binding protein expressed in the epidermis and related to the calmodulin family of calcium binding proteins. Functional studies with recombinant protein demonstrate it does bind calcium and undergoes a conformational change when it does so. Abundant expression is detected only in reconstructed epidermis and is restricted to differentiating keratinocytes. In addition, it can associate with transglutaminase 3, shown to be a key enzyme in the terminal differentiation of keratinocytes. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-146 of human CALML5 (NP_059118.2). |
Gene ID: | 51806 |
Swiss prot: | Q9NZT1 |
Molecular weight: | 15kDa |
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