Western blot analysis of extracts of various cell lines, using CA5B antibody (A17633) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
CA5B Rabbit pAb
Catalog No:AB-A17633
Manufacturer:ABclonal
Synonyms:CA5B;CA-VB
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | CA5B Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA VA. It has a wider tissue distribution than CA VA, which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles. [provided by RefSeq, Jul 2008] |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human CA5B (NP_009151.1). |
Gene ID: | 11238 |
Swiss prot: | Q9Y2D0 |
Molecular weight: | 36kDa |
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