Western blot analysis of extracts of HT-29 cells, using CA12 antibody (A10187) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.
CA12 Rabbit pAb
Catalog No:AB-A10187
Manufacturer:ABclonal
Synonyms:CA12;CA-XII;CAXII;HsT18816;T18816
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | CA12 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 25-301 of human CA12 (NP_001209.1). |
Gene ID: | 771 |
Swiss prot: | O43570 |
Molecular weight: | 38kDa/39kDa |
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