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Western blot analysis of extracts of HeLa cells, using c-Fos antibody (A16641) at 1:1000 dilution.HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
c-Fos Rabbit pAb
Catalog No：AB-A16641
Manufacturer：ABclonal
Synonyms：FOS;AP-1;C-FOS;p55;c-Fos
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | c-Fos Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. |
| Immunogen: | Recombinant protein of human c-Fos |
| Gene ID: | 2353 |
| Swiss prot: | P01100 |
| Molecular weight: | 28kDa/36kDa/40kDa |
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