Western blot analysis of extracts of various cell lines, using BUB3 antibody (A6536) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
BUB3 Rabbit pAb
Catalog No:AB-A6536
Manufacturer:ABclonal
Synonyms:BUB3;BUB3L;hBUB3
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | BUB3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human BUB3 (NP_004716.1). |
Gene ID: | 9184 |
Swiss prot: | O43684 |
Molecular weight: | 36kDa/37kDa |
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