Western blot analysis of extracts of various cell lines, using BTD antibody (A6284) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
BTD Rabbit pAb
Catalog No:AB-A6284
Manufacturer:ABclonal
Synonyms:BTD
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | BTD Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. The encoded protein has also been shown to have biotinyl transferase activity. Mutations in this gene are associated with biotinidase deficiency. Multiple transcript variants encoding different isoforms have been described. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 284-543 of human BTD (NP_000051.1). |
Gene ID: | 686 |
Swiss prot: | P43251 |
Molecular weight: | 58kDa/61kDa |
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