Western blot analysis of extracts of various cell lines, using BRD4 antibody (A2249) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
BRD4 Rabbit pAb
Catalog No:AB-A2249
Manufacturer:ABclonal
Synonyms:BRD4;CAP;HUNK1;HUNKI;MCAP
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | BRD4 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1100-1200 of human BRD4 (NP_490597.1). |
Gene ID: | 23476 |
Swiss prot: | O60885 |
Molecular weight: | 80kDa/88kDa/152kDa |
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