Western blot analysis of extracts of various cell lines, using BOLA3 antibody (A15985) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
BOLA3 Rabbit pAb
Catalog No:AB-A15985
Manufacturer:ABclonal
Synonyms:BOLA3;MMDS2
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | BOLA3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a protein that plays an essential role in the production of iron-sulfur (Fe-S) clusters for the normal maturation of lipoate-containing 2-oxoacid dehydrogenases, and for the assembly of the mitochondrial respiratory chain complexes. Mutation in this gene has been associated with multiple mitochondrial dysfunctions syndrome-2. Two alternatively spliced transcript variants encoding different isoforms with distinct subcellular localization have been reported for this gene (PMID:21944046). |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-68 of human BOLA3 (NP_997717.2). |
Gene ID: | 388962 |
Swiss prot: | Q53S33 |
Molecular weight: | 11kDa/12kDa |
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