Western blot analysis of extracts of various cell lines, using BNIP3 antibody (A19593) at 1:1000 dilution.Hela cells and NIH/3T3 cells were treated by Cobalt chloride (0.1 mM) at 37℃ for 4 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
BNIP3 Rabbit mAb
Catalog No:AB-A19593
Manufacturer:ABclonal
Synonyms:BNIP3;NIP3
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | BNIP3 Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation. [provided by RefSeq, Dec 2014] |
Immunogen: | A synthesized peptide derived from human BNIP3. |
Gene ID: | 664 |
Swiss prot: | Q12983 |
Molecular weight: | 30kDa |
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