BglII
Catalog No：AB-RK21165
Manufacturer：ABclonal
Applications：qPCR
€37,00 – €150,00
Description
5’…A/GATCT…3′
3’…TCTAG/A…5′
3’…TCTAG/A…5′
Product Components
| Components | Cat. No. |
|---|---|
| BglII (20, 000 U/ml) | AB-RM21665 |
| 10X Buffer CutU | AB-RM20102 |
Product Information
| Featured | Specification | ||||||
|---|---|---|---|---|---|---|---|
| Applications | Restriction Enzyme Digestion | ||||||
| Unit Definition | One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (HindIII digest) fragments in 1 hour at 37°C in a total reaction volume of 50 µl. | ||||||
| Storage Conditions | 10 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200 μg/ml BSA, 50% Glycerol, pH 7.4 @ 25°C | ||||||
| Storage Temperature | -20°C | ||||||
| Reaction Conditions | 1X Buffer CutU. Incubate at 37°C. | ||||||
| Reaction Buffer or Mix | 1X Buffer CutU: 20 mM Tris-acetate，10 mM Magnesium acetate，50 mM Potassium acetate，100 µg/ml BSA，pH 7.9 @25℃ |
||||||
| Heat Inactivation | 80°C for 20 min | ||||||
| Methylation Sensitivity |
|
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