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Western blot analysis of extracts of various cell lines, using Bcl-2 antibody (A0208) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human appendix using Bcl-2 Rabbit pAb (A0208) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of BALB-3T3 cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of C6 cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded human appendix using Bcl-2 Rabbit pAb (A0208) at dilution of 1:100 (40x lens).
Bcl-2 Rabbit pAb
Catalog No：AB-A0208
Manufacturer：ABclonal
Synonyms：Bcl-2;PPP1R50;BCL2
Applications：WB,IHC,IF,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €1.760,00
| Product Name: | Bcl-2 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. |
| Immunogen: | A synthetic peptide of human Bcl-2 |
| Gene ID: | 596 |
| Swiss prot: | P10415 |
| Molecular weight: | 22kDa/26kDa |
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