Western blot analysis of extracts of various cell lines, using BAG2 antibody (A7763) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 60s.
BAG2 Rabbit pAb
Catalog No:AB-A7763
Manufacturer:ABclonal
Synonyms:BAG2;BAG-2;dJ417I1.2
Applications:WB,IHC,IF
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | BAG2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human BAG2 (NP_004273.1). |
Gene ID: | 9532 |
Swiss prot: | O95816 |
Molecular weight: | 20kDa/23kDa |
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