Western blot analysis of extracts of various cell lines, using ATG7 antibody (A0691) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
ATG7 Rabbit pAb
Catalog No:AB-A0691
Manufacturer:ABclonal
Synonyms:ATG7;APG7-LIKE;APG7L;GSA7;Apg7/Atg7
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | ATG7 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 500-676 of human ATG7 (NP_001129503.2). |
Gene ID: | 10533 |
Swiss prot: | O95352 |
Molecular weight: | 68kDa/75kDa/77kDa |
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