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Western blot analysis of extracts of various cell lines, using ATG12 antibody (A15609) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
ATG12 Rabbit pAb
Catalog No：AB-A15609
Manufacturer：ABclonal
Synonyms：ATG12;APG12;APG12L;FBR93;HAPG12;Apg12
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | ATG12 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]). |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-74 of human ATG12 (NP_001264712.1). |
| Gene ID: | 9140 |
| Swiss prot: | O94817 |
| Molecular weight: | 7kDa/15kDa |
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