Western blot analysis of extracts of various cell lines, using ATG10 Rabbit mAb (A6848) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
ATG10 Rabbit mAb
Catalog No:AB-A6848
Manufacturer:ABclonal
Synonyms:APG10, APG10L, pp12616
Applications:WB,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | ATG10 Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM, Mar 2008] |
Immunogen: | A synthesized peptide derived from human ATG10 |
Gene ID: | 83734 |
Swiss prot: | Q9H0Y0 |
Molecular weight: | 25kDa |
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