Western blot analysis of extracts of various cell lines, using ASPN antibody (A10311) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
ASPN Rabbit pAb
Catalog No:AB-A10311
Manufacturer:ABclonal
Synonyms:ASPN;OS3;PLAP-1;PLAP1;SLRR1C;asporin
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | ASPN Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a cartilage extracellular protein that is member of the small leucine-rich proteoglycan family. The encoded protein may regulate chondrogenesis by inhibiting transforming growth factor-beta 1-induced gene expression in cartilage. This protein also binds collagen and calcium and may induce collagen mineralization. Polymorphisms in the aspartic acid repeat region of this gene are associated with a susceptibility to osteoarthritis, and also with intervertebral disc disease. Alternative splicing of this gene results in multiple transcript variants. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-379 of human ASPN (NP_060150.4). |
Gene ID: | 54829 |
Swiss prot: | Q9BXN1 |
Molecular weight: | 43kDa |
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