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Western blot analysis of extracts of various cell lines, using ASPH antibody (A13153) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
ASPH Rabbit pAb
Catalog No：AB-A13153
Manufacturer：ABclonal
Synonyms：ASPH;AAH;BAH;CASQ2BP1;FDLAB;HAAH;JCTN;junctin
Applications：WB,IHC
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | ASPH Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene is thought to play an important role in calcium homeostasis. The gene is expressed from two promoters and undergoes extensive alternative splicing. The encoded set of proteins share varying amounts of overlap near their N-termini but have substantial variations in their C-terminal domains resulting in distinct functional properties. The longest isoforms (a and f) include a C-terminal Aspartyl/Asparaginyl beta-hydroxylase domain that hydroxylates aspartic acid or asparagine residues in the epidermal growth factor (EGF)-like domains of some proteins, including protein C, coagulation factors VII, IX, and X, and the complement factors C1R and C1S. Other isoforms differ primarily in the C-terminal sequence and lack the hydroxylase domain, and some have been localized to the endoplasmic and sarcoplasmic reticulum. Some of these isoforms are found in complexes with calsequestrin, triadin, and the ryanodine receptor, and have been shown to regulate calcium release from the sarcoplasmic reticulum. Some isoforms have been implicated in metastasis. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 111-270 of human ASPH (NP_001158227.1). |
| Gene ID: | 444 |
| Swiss prot: | Q12797 |
| Molecular weight: | 21-34kDa/83-85kDa |
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