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Western blot analysis of extracts of various cell lines, using ARFGEF1 antibody (A16483) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
ARFGEF1 Rabbit pAb
Catalog No：AB-A16483
Manufacturer：ABclonal
Synonyms：ARFGEF1;ARFGEP1;BIG1;P200
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | ARFGEF1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP. It contains a Sec7 domain, which may be responsible for guanine-nucleotide exchange activity and also brefeldin A inhibition. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 220-420 of human ARFGEF1 (NP_006412.2). |
| Gene ID: | 10565 |
| Swiss prot: | Q9Y6D6 |
| Molecular weight: | 208kDa |
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