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Western blot analysis of extracts of various cell lines, using ARFGAP1 antibody (A12595) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
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Immunohistochemistry of paraffin-embedded rat brain using ARFGAP1 antibody (A12595) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using ARFGAP1 antibody (A12595) at dilution of 1:100 (40x lens).
ARFGAP1 Rabbit pAb
Catalog No：AB-A12595
Manufacturer：ABclonal
Synonyms：ARFGAP1;ARF1GAP;HRIHFB2281
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | ARFGAP1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ARFGAP1 (NP_001268411.1). |
| Gene ID: | 55738 |
| Swiss prot: | Q8N6T3 |
| Molecular weight: | 31kDa/39kDa/44kDa/45kDa |
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