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Western blot analysis of extracts of various cell lines, using ApoB antibody (A1330) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
ApoB Rabbit pAb
Catalog No：AB-A1330
Manufacturer：ABclonal
Synonyms：APOB;FLDB;LDLCQ4;apoB-100;apoB-48
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | ApoB Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 28-330 of human ApoB (NP_000375.2). |
| Gene ID: | 338 |
| Swiss prot: | P04114 |
| Molecular weight: | 515kDa |
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