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Western blot analysis of extracts of various cell lines, using ANAPC5 antibody (A7109) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat kidney using ANAPC5 antibody (A7109) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human kidney using ANAPC5 antibody (A7109) at dilution of 1:100 (40x lens).
ANAPC5 Rabbit pAb
Catalog No：AB-A7109
Manufacturer：ABclonal
Synonyms：ANAPC5;APC5
Applications：WB,IHC,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | ANAPC5 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ANAPC5 (NP_057321.2). |
| Gene ID: | 51433 |
| Swiss prot: | Q9UJX4 |
| Molecular weight: | 25kDa/72kDa/85kDa |
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