Western blot analysis of extracts of various cell lines, using AMPKa1/AMPKa2 antibody (A17290) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Western blot analysis of extracts of Mouse liver, using AMPKa1/AMPKa2 antibody (A17290) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
AMPKa1/AMPKa2 Rabbit pAb
Catalog No:AB-A17290
Manufacturer:ABclonal
Synonyms:AMPKa1/AMPKa2
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | AMPKa1/AMPKa2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | AMPK belongs to the ser/thr protein kinase family. Alpha subunit is the catalytic subunit of the 5′-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. |
Immunogen: | Recombinant protein of human AMPKa1/AMPKa2. |
Gene ID: | 5562/5563 |
Swiss prot: | Q13131/P54646 |
Molecular weight: | 64kDa/65kDa |
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