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Western blot analysis of extracts of MCF7 cells, using AMPKβ1 Rabbit pAb (A15708) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
AMPKβ1 Rabbit pAb
Catalog No：AB-A15708
Manufacturer：ABclonal
Synonyms：PRKAB1;AMPK;HAMPKb
Applications：WB,IF
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | AMPKβ1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human AMPKβ1 (NP_006244.2). |
| Gene ID: | 5564 |
| Swiss prot: | Q9Y478 |
| Molecular weight: | 30kDa |
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