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Western blot analysis of extracts of various cell lines, using Alpha-Fetoprotein (Alpha-Fetoprotein (AFP)) antibody (A0200) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 40s.
Alpha-Fetoprotein (AFP) Rabbit pAb
Catalog No：AB-A0200
Manufacturer：ABclonal
Synonyms：AFPD;FETA;HPAFP;AFP
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | Alpha-Fetoprotein (AFP) Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 360-609 of human Alpha-Fetoprotein (Alpha-Fetoprotein (AFP)) (NP_001125.1). |
| Gene ID: | 174 |
| Swiss prot: | P02771 |
| Molecular weight: | 68kDa |
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