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Western blot analysis of extracts of various cell lines, using alpha 1 Spectrin antibody (A12355) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded human tonsil using alpha 1 Spectrin antibody (A12355) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat spleen using alpha 1 Spectrin antibody (A12355) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using alpha 1 Spectrin antibody (A12355) at dilution of 1:100 (40x lens).
alpha 1 Spectrin Rabbit pAb
Catalog No：AB-A12355
Manufacturer：ABclonal
Synonyms：SPTA1;EL2;HPP;HS3;SPH3;SPTA
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | alpha 1 Spectrin Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is a tetramer made up of alpha-beta dimers linked in a head-to-head arrangement. This gene is one member of a family of alpha-spectrin genes. The encoded protein is primarily composed of 22 spectrin repeats which are involved in dimer formation. It forms weaker tetramer interactions than non-erythrocytic alpha spectrin, which may increase the plasma membrane elasticity and deformability of red blood cells. Mutations in this gene result in a variety of hereditary red blood cell disorders, including elliptocytosis type 2, pyropoikilocytosis, and spherocytic hemolytic anemia. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 940-1160 of human alpha 1 Spectrin (NP_003117.2). |
| Gene ID: | 6708 |
| Swiss prot: | P02549 |
| Molecular weight: | 279kDa/280kDa |
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