Western blot analysis of extracts of various cell lines, using alpha 1 Spectrin Rabbit mAb (A9597) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
alpha 1 Spectrin Rabbit mAb
Catalog No:AB-A9597
Manufacturer:ABclonal
Synonyms:EL2, HPP, HS3, SPH3, SPTA
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | alpha 1 Spectrin Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is a tetramer made up of alpha-beta dimers linked in a head-to-head arrangement. This gene is one member of a family of alpha-spectrin genes. The encoded protein is primarily composed of 22 spectrin repeats which are involved in dimer formation. It forms weaker tetramer interactions than non-erythrocytic alpha spectrin, which may increase the plasma membrane elasticity and deformability of red blood cells. Mutations in this gene result in a variety of hereditary red blood cell disorders, including elliptocytosis type 2, pyropoikilocytosis, and spherocytic hemolytic anemia. [provided by RefSeq, Jul 2008] |
Immunogen: | A synthesized peptide derived from human alpha 1 Spectrin |
Gene ID: | 6708 |
Swiss prot: | P02549 |
Molecular weight: | 280kDa |
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