Western blot analysis of extracts of various cell lines, using ALDOA antibody (A1142) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 2s.
ALDOA Rabbit pAb
Catalog No:AB-A1142
Manufacturer:ABclonal
Synonyms:ALDOA;ALDA;GSD12;HEL-S-87p
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | ALDOA Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene, Aldolase A (fructose-bisphosphate aldolase), is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing and alternative promoter usage results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 10. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-348 of human ALDOA (NP_908930.1). |
Gene ID: | 226 |
Swiss prot: | P04075 |
Molecular weight: | 39kDa/45kDa |
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