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Western blot analysis of extracts of various cell lines, using ALAS1 antibody (A6521) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 20s.
ALAS1 Rabbit pAb
Catalog No：AB-A6521
Manufacturer：ABclonal
Synonyms：ALAS1;ALAS;ALAS-H;ALAS3;ALASH;MIG4
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | ALAS1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ALAS1 (NP_954635.1). |
| Gene ID: | 211 |
| Swiss prot: | P13196 |
| Molecular weight: | 12kDa/70kDa |
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