Western blot analysis of extracts of various cell lines, using ALAS1 Rabbit mAb (A4817) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
ALAS1 Rabbit mAb
Catalog No:AB-A4817
Manufacturer:ABclonal
Synonyms:ALAS, ALAS-H, ALAS3, ALASH, MIG4
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | ALAS1 Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2015] |
Immunogen: | A synthesized peptide derived from human ALAS1 |
Gene ID: | 211 |
Swiss prot: | P13196 |
Molecular weight: | 71kDa |
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