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Western blot analysis of extracts of various cell lines, using ALAD antibody (A12395) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
ALAD Rabbit pAb
Catalog No：AB-A12395
Manufacturer：ABclonal
Synonyms：ALAD;ALADH;PBGS
Applications：WB,IF
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | ALAD Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human ALAD (NP_000022.3). |
| Gene ID: | 210 |
| Swiss prot: | P13716 |
| Molecular weight: | 36kDa/39kDa |
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