Western blot analysis of extracts of various cell lines, using AKT1S1/PRAS40 antibody (A6238) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
AKT1S1/PRAS40 Rabbit pAb
Catalog No:AB-A6238
Manufacturer:ABclonal
Synonyms:AKT1S1;Lobe;PRAS40
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | AKT1S1/PRAS40 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008] |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 152-276 of human AKT1S1/PRAS40 (NP_115751.3). |
Gene ID: | 84335 |
Swiss prot: | Q96B36 |
Molecular weight: | 13kDa/27kDa/29kDa |
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