Western blot analysis of extracts of various cell lines, using AKR1C4 antibody (A7430) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
AKR1C4 Rabbit pAb
Catalog No:AB-A7430
Manufacturer:ABclonal
Synonyms:AKR1C4;3-alpha-HSD;C11;CDR;CHDR;DD-4;DD4;HAKRA
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | AKR1C4 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human AKR1C4 (NP_001809.3). |
Gene ID: | 1109 |
Swiss prot: | P17516 |
Molecular weight: | 37kDa |
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