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Western blot analysis of extracts of various cell lines, using AHA1 Antibody (A8018) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
AHA1 Rabbit pAb
Catalog No：AB-A8018
Manufacturer：ABclonal
Synonyms：AHSA1;AHA1;C14orf3;hAha1;p38
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | AHA1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | AHA-1 stimulates the inherent ATPase activity of yeast and human HSP 90 and interacts with the cytoplasmic tail of vesticular stomatitis virus glycoprotein. AHA-1 regulates HSP 90 by influencing the conformational state of the 鈥淎TP lid鈥 and consequent N-terminal dimerization. It is crucial for cell viability under non-optimal growth conditions when HSP 90 levels are limiting. AHA-1 is a cytosolic protein and may transiently interact with the endoplasmic reticulum. It can have an affect on one step in the endoplasmic to Golgi trafficking. AHA-1 is expressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, in liver and placenta. It is induced by heat shock and treatment with the HSP 90 inhibitor 17-demeth-oxygeldanamycin. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-338 of human AHA1 (NP_036243.1). |
| Gene ID: | 10598 |
| Swiss prot: | O95433 |
| Molecular weight: | 32kDa/38kDa |
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