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Western blot analysis of extracts of various cell lines, using AGO1 antibody (A6022) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
AGO1 Rabbit pAb
Catalog No：AB-A6022
Manufacturer：ABclonal
Synonyms：AGO1;EIF2C;EIF2C1;GERP95;Q99;hAgo1
Applications：WB,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | AGO1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human AGO1 (NP_036331.1). |
| Gene ID: | 26523 |
| Swiss prot: | Q9UL18 |
| Molecular weight: | 97kDa |
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