AgeI
Catalog No：AB-RK21125
Manufacturer：ABclonal
Applications：Restriction Endonucleases
€40,00 – €160,00
Description
3’…TGGCC/A…5′
Product Components
| Components | Cat. No. |
|---|---|
| AgeI (5, 000 U/ml) | AB-RM21626 |
| 10X Buffer CutA | AB-RM20104 |
Product Information
| Featured | Specification | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Applications | Restriction Enzyme Digestion | ||||||||
| Unit Definition | One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction of 50 µl. | ||||||||
| Storage Conditions | 10 mM Tris-HCl, 250 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200 μg/ml BSA, 50% Glycerol, 0.15% Triton X-100, pH 7.4 @ 25°C | ||||||||
| Storage Temperature | -20°C | ||||||||
| Reaction Conditions | 1X Buffer CutA, Incubate at 37°C. | ||||||||
| Reaction Buffer or Mix | 1X Buffer CutA: 10 mM Bis-Tris-Propane-HCl, 10 mM MgCl2, 100 μg/ml BSA, pH 7 @ 25°C |
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| Heat Inactivation | 65°C for 20 min | ||||||||
| Methylation Sensitivity |
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| Activity in Abclonal Buffers |
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