Western blot analysis of extracts of various cell lines, using AFF4 antibody (A4644) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 30s.
AFF4 Rabbit pAb
Catalog No:AB-A4644
Manufacturer:ABclonal
Synonyms:AFF4;AF5Q31;CHOPS;MCEF
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | AFF4 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. A chromosomal translocation involving this gene and MLL gene on chromosome 11 is found in infant acute lymphoblastic leukemia with ins(5;11)(q31;q31q23). |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human AFF4 (NP_055238.1). |
Gene ID: | 27125 |
Swiss prot: | Q9UHB7 |
Molecular weight: | 38kDa/98kDa/127kDa |
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