Western blot analysis of extracts of various cell lines, using ACTL6A antibody (A5387) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
ACTL6A Rabbit pAb
Catalog No:AB-A5387
Manufacturer:ABclonal
Synonyms:ACTL6A;ACTL6;ARPN-BETA;Arp4;BAF53A;INO80K
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | ACTL6A Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ACTL6A (NP_004292.1). |
Gene ID: | 86 |
Swiss prot: | O96019 |
Molecular weight: | 43kDa/47kDa |
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