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Western blot analysis of extracts of various cell lines, using Acetyl-p53- K370 antibody (A19836) at 1:1000 dilution.HeLa cells and NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 1s.
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Western blot analysis of extracts of C6 cells, using Acetyl-p53- K370 antibody (A19836) at 1:1000 dilution.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Acetyl-p53- K370 Rabbit mAb
Catalog No：AB-A19836
Manufacturer：ABclonal
Synonyms：BCC7, LFS1, P53, TRP53
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Acetyl-p53- K370 Rabbit mAb |
| Category: | Acetylated Antibodies |
| Description: | This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016] |
| Immunogen: | A synthetic acetylated peptide around K370 of human p53 |
| Gene ID: | 7157 |
| Swiss prot: | P04637 |
| Molecular weight: | 53kDa |
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