Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K5 antibody (A15233) at 1:1000 dilution. HeLa cells were treated by TSA (1uM) for 18 hours. C6 cells were treated by TSA (1uM) for 18 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Acetyl-Histone H4-K5 Rabbit pAb
Catalog No:AB-A15233
Manufacturer:ABclonal
Synonyms:FO108;H4;H4/n;H4F2;H4FN;HIST2H4;Histone H4;HIST1H4A;HIST2H4A
Applications:WB,IHC,IF,ChIP
Reactivity:Human,Mouse,Rat,Other (Wide Range)
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Acetyl-Histone H4-K5 Rabbit pAb |
Category: | Acetylated Antibodies |
Description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. |
Immunogen: | A synthetic acetylated peptide around K5 of human Histone H4 (NP_001029249.1). |
Gene ID: | 8370 |
Swiss prot: | P62805 |
Molecular weight: | 11kDa |
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