Western blot analysis of extracts of HeLa cells, using Acetyl-Histone H3-K4 antibody (A17019) at 1:500 dilution.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 300s.
Acetyl-Histone H3-K4 Rabbit pAb
Catalog No:AB-A17019
Manufacturer:ABclonal
Synonyms:H3.4;H3/g;H3FT;H3t;HIST3H3;Histone H3;HIST1H3A
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €325,00
Product Name: | Acetyl-Histone H3-K4 Rabbit pAb |
Category: | Acetylated Antibodies |
Description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. |
Immunogen: | A synthetic acetylated peptide around K4 of human Histone H3 (NP_003484.1). |
Gene ID: | 8290 |
Swiss prot: | Q16695 |
Molecular weight: | 15kDa |
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