Western blot analysis of extracts of various cell lines, using ACC1 antibody (A20183) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
ACC1 Rabbit pAb
Catalog No:AB-A20183
Manufacturer:ABclonal
Synonyms:ACC; ACAC; ACC1; ACCA; ACACAD
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€125,00 – €370,00
Product Name: | ACC1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5′ sequence and encoding distinct isoforms have been found for this gene. |
Immunogen: | Recombinant protein of human ACACA. |
Gene ID: | 31 |
Swiss prot: | Q13085 |
Molecular weight: | 265kDa |
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