Western blot analysis of extracts of HeLa cells, using ABL1 antibody (A0282) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
ABL1 Rabbit pAb
Catalog No:AB-A0282
Manufacturer:ABclonal
Synonyms:ABL;JTK7;p150;c-ABL;v-abl;CHDSKM;c-ABL1;ABL1;c-Abl;bcr/abl
Applications:WB,IHC,IF
Reactivity:Human,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | ABL1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5′ end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 490-590 of human ABL1 (NP_005148.2). |
Gene ID: | 25 |
Swiss prot: | P00519 |
Molecular weight: | 122kDa/124kDa |
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