Western blot analysis of extracts of various cell lines, using β-Actin antibody (AC038) at 1:50000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Western blot analysis of extracts of HeLa cells, using β-Actin antibody (AC038) at 1:10000-1:320000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
β-Actin Rabbit mAb
Catalog No:AB-AC038
Manufacturer:ABclonal
Synonyms:ACTB;BRWS1;PS1TP5BP1;beta actin;actin
Applications:WB,IHC
Reactivity:Human,Mouse,Rat,Chicken,Zebrafish,Pig,Oryza sativa
Host:Rabbit
Conjugate:No
€70,00 – €185,00
Product Name: | 尾-Actin Rabbit mAb |
Category: | Tag and Loading Control Antibodies |
Description: | This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Put 5ul AC038 into 45 ul buffer(with 50% glycerol), diluted 1:5,000 for using. The diluted antibody can be stored at -20鈩 without aliquot. |
Immunogen: | Recombinant protein of human 尾-Actin |
Gene ID: | 60 |
Swiss prot: | P60709 |
Molecular weight: | 41kDa |
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