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Western blot analysis of extracts of various cell lines, using alpha Tubulin antibody (AC012) at 1:5000 dilution.|Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
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Immunofluorescence analysis of C6 cells using ɑ-Tubulin Mouse mAb (AC012) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using ɑ-Tubulin Mouse mAb (AC012) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2 OS cells using ɑ-Tubulin Mouse mAb (AC012) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunohistochemistry of paraffin-embedded mouse brain using ɑ-Tubulin Mouse mAb (AC012) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using ɑ-Tubulin Mouse mAb (AC012) at dilution of 1:100 (40x lens).
α-Tubulin Mouse mAb
Catalog No：AB-AC012
Manufacturer：ABclonal
Synonyms：TUBA4A;ALS22;H2-ALPHA;TUBA1
Applications：WB,IHC,IF,IP
Reactivity：Human,Mouse,Rat
Host：Mouse
Conjugate：No
€85,00 – €255,00
| Product Name: | 伪-Tubulin Mouse mAb |
| Category: | Tag and Loading Control Antibodies |
| Description: | Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | Recombinant protein of human TUBA1B |
| Gene ID: | 7277 |
| Swiss prot: | P68366 |
| Molecular weight: | 48kDa/49kDa |
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