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Western blot analysis of extracts of various cell lines, using α-Actinin-4 Rabbit mAb (A3379) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
α-Actinin-4 Rabbit mAb
Catalog No：AB-A3379
Manufacturer：ABclonal
Synonyms：ACTININ-4, FSGS, FSGS1
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | 伪-Actinin-4 Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq, Jul 2008] |
| Immunogen: | A synthesized peptide derived from human 伪-Actinin-4 |
| Gene ID: | 81 |
| Swiss prot: | O43707 |
| Molecular weight: | 105kDa |
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